Culture medium and other recipes

Modified Neff medium

0.25% proteose peptone
0.25% yeast extract
0.5% glucose
33.3 ╬╝M FeCl3

To avoid precipitate formation, the FeCl3 is first dissolved in one quarter of the final H2O volume, and the glucose, yeast and proteose peptone are added and dissolved next. The remainder of the H2O is then added, bottled and autoclaved.

See: Cassidy-Hanley D, Bowen J, Lee JH, Cole E, VerPlank LA, Gaertig J, Gorovsky MA, Bruns PJ. 1997. Germline and somatic transformation of mating tetrahymena thermophila by particle bombardment. Genetics 146(1):135-47.